Terms needed: Any - 10 weeks
Faculty mentor name: Aymeric Goyer
BES Facility: HAREC
Location (town) of internship: Hermiston
Hourly Salary: $12 Expected hours/week: 40
Student may be asked to work on weekends or outside of 8-5
Are Housing Benefits included in addition to hourly salary? no
My group is trying to understand the mechanism of resistance against Potato Virus Y (PVY) at the molecular level in potato. In this project, we will characterize the function of genes that are good candidates in the establishment of the resistance to PVY. The most efficient approach to demonstrate what genes do is to disable them and analyze the consequences on the plant’s behavior, in this case resistance against PVY. This proposal aims to disable genes that are candidate components of the resistance mechanism against PVY, and then characterize disabled plants for resistance to PVY. In order to disable candidate genes, we will use the new genome editing technology called CRISPR/Cas9. This technology promises to revolutionize the development of improved crops by enabling precise targeted manipulation of specific genomic sequences, compared to current breeding approaches which are completely random and laborious. In this project, we will create mutations to disrupt the targeted genes by using CRISPR/Cas9.
For this project, the student intern will be expected to spend 40 hours per week in a laboratory. The student will assist a Master graduate student. Laboratory work will consist of preparing media for plant tissue culture and Agrobacterium-mediated transformation of potato, propagating in vitro plantlets, and assisting in Agrobacterium-mediated transformation. In addition, the student will be responsible for collecting leaf sample, extract DNA, and use PCR to genotype potato transformants. Intern must be able to work both independently and as part of a team. Strong organizational and communication skills are required.