TitleIdentification and Characterization of GPCRs for Pyrokinin and CAPA Peptides in the Brown Marmorated Stink Bug, (Hemiptera: Pentatomidae).
Publication TypeJournal Article
Year of Publication2020
AuthorsAhn, S-J, Corcoran, JA, Meer, RKVander, Choi, M-Y
JournalFront Physiol
Volume11
Pagination559
Date Published2020
ISSN1664-042X
Abstract

The brown marmorated stink bug, , is an invasive hemipteran that causes significant economic losses to various agricultural products around the world. Recently, the and genes that express multiple neuropeptides were described in this species. Here we report six and GPCRs including two splice variants, and evaluate their (a) ability to respond to neuropeptides in cell-based assays, and (b) expression levels by RT-PCR. Functional studies revealed that the pyrokinin receptor-1 (HalhaPK-R1a & b) responded to the pyrokinin 2 (PK2) type peptide. RT-PCR results revealed that these receptors had little or no expression in the tissues tested, including the whole body, central nervous system, midgut, Malpighian tubules, and reproductive organs of males and females. HalhaPK-R2 showed the strongest response to PK2 peptides and a moderate response to pyrokinin 1 (PK1) type peptides (= DH, diapause hormone), and was expressed in all tissues tested. HalhaPK-R3a & b responded to both PK1 and PK2 peptides. Their gene expression was restricted mostly to the central nervous system and Malpighian tubules. All PK receptors were dominantly expressed in the fifth nymph. HalhaCAPA-R responded specifically to CAPA-PVK peptides (PVK1 and PVK2), and was highly expressed in the Malpighian tubules with low to moderate expression in other tissues, and life stages. Of the six GPCRs, HalhaPK-R3b showed the strongest response to PK1. Our experiments associated the following peptide ligands to the six GPCRs: HalhaPK-R1a & b and HalhaPK-R2 are activated by PK2 peptides, HalhaPK-R3a & b are activated by PK1 (= DH) peptides, and HalhaCAPA-R is activated by PVK peptides. These results pave the way for investigations into the biological functions of PK and CAPA peptides, and possible species-specific management of .

DOI10.3389/fphys.2020.00559
Alternate JournalFront Physiol
PubMed ID32547421
PubMed Central IDPMC7274154