|Intracellular free iron and its potential role in ultrahigh-pressure-induced inactivation of Escherichia coli.
|Year of Publication
|Yan, Y, Waite-Cusic, JG, Kuppusamy, P, Yousef, AE
|Appl Environ Microbiol
|Electron Spin Resonance Spectroscopy, Escherichia coli, Hydrostatic Pressure, Iron, Microbial Viability
Intracellular free iron of Escherichia coli was determined by whole-cell electron paramagnetic resonance spectrometry. Ultrahigh pressure (UHP) increased both intracellular free iron and cell lethality in a pressure-dose-dependent manner. The iron chelator 2,2'-dipyridyl protected cells against UHP treatments. A mutation that produced iron overload conditions sensitized E. coli to UHP treatment.
|Appl. Environ. Microbiol.
|PubMed Central ID