Title | Preparation, characterization and evaluation of antibacterial activity of catechins and catechins-Zn complex loaded β-chitosan nanoparticles of different particle sizes. |
Publication Type | Journal Article |
Year of Publication | 2016 |
Authors | Zhang, H, Jung, J, Zhao, Y |
Journal | Carbohydr Polym |
Volume | 137 |
Pagination | 82-91 |
Date Published | 2016 Feb 10 |
ISSN | 1879-1344 |
Keywords | Anti-Bacterial Agents, Catechin, Chitosan, Escherichia coli, Listeria, Microbial Sensitivity Tests, Nanoparticles, Particle Size, Zinc |
Abstract | This study used β-chitosan nanoparticles (β-CS NPs) of different particle sizes to encapsulate catechins (CAT) or CAT-Zn complex by ionic gelation technology. The antibacterial activity of CAT or CAT-Zn complex loaded β-CS NPs against Escherichia coli and Listeria innocua were investigated based on bacterial growth curve, minimum inhibitory concentration (MIC), and minimum bacterial concentration (MBC). Fourier transform infrared spectrometer (FT-IR) was employed to study the incorporation of CAT or CAT-Zn complex into β-CS NPs. The CAT-Zn complex loaded β-CS NPs had particle size of 208-591 nm, polydispersity index (PDI) of 0.377-0.395, and positive Zeta-potential of 39.17-45.62 mV. The CAT-Zn complex loaded β-CS NPs of smaller particle sizes showed higher antibacterial activity than that of larger particle size ones. The MIC and MBC of CAT-Zn complex loaded β-CS NPs of the smallest particle size against L. innocua and E. coli were 0.031 and 0.063 mg/mL, and 0.063 and 0.125 mg/mL, respectively. This study suggested that encapsulation of CAT-Zn complex in β-CS NPs improved the antibacterial activity of CAT and CAT-Zn complex, and the encapsulators have great potential to be used as antibacterial substances for food and other applications through either direct addition or incorporation into packaging materials. |
DOI | 10.1016/j.carbpol.2015.10.036 |
Alternate Journal | Carbohydr Polym |
PubMed ID | 26686108 |