TitleThe role of chorion on toxicity of silver nanoparticles in the embryonic zebrafish assay.
Publication TypeJournal Article
Year of Publication2014
AuthorsKim, K-T, Tanguay, RL
JournalEnviron Health Toxicol
Volume29
Paginatione2014021
Date Published2014
ISSN2233-6567
Abstract

OBJECTIVES: This study was designed to investigate how the size- and surface coating-dependent toxicity of silver nanoparticles (AgNPs) is influenced by the presence and absence of the chorion in an embryonic zebrafish assay.

METHODS: Normal and dechorinated embryos were exposed to four different AgNPs, 20 or 110 nm in size, with polypyrrolidone (PVP) or citrate surface coatings in a standard zebrafish embryo medium (EM). This was then compared to a 62.5 μM calcium chloride (CaCl2) solution where agglomeration was controlled.

RESULTS: Embryonic toxicity in the absence of the chorion was greater than in its presence. The smaller 20 nm AgNPs were more toxic than the larger 110 nm AgNPs, regardless of the chorion and test media. However, surface coating affected toxicity, since PVPcoated AgNPs were more toxic than citrate-coated AgNPs; this was strongly affected by the presence of the chorion in both EM and CaCl2.

CONCLUSIONS: Our results demonstrate the permeability function of the chorion on the size- and surface coating-dependent toxicity of AgNPs. Thereafter, careful experiment should be conducted to assess nanoparticle toxicity in zebrafish embryos.

DOI10.5620/eht.e2014021
Alternate JournalEnviron Health Toxicol
PubMed ID25518841
PubMed Central IDPMC4300859